Key Responsibilities:
• Protein Expression: Transfection of plasmids into mammalian cells to express key viral proteins.
• Protein Purification: Design, optimize, and execute protocols for the purification of recombinant proteins using various chromatography techniques (e.g., affinity, ion exchange, size exclusion).
• Biophysical Characterization: Perform biophysical characterization of purified proteins, including but not limited to, SDS-PAGE, analytical SEC, and Differential Scanning Fluorimetry (DSF).
• Data Analysis & Reporting: Maintain detailed records of experimental designs, data, and results. Analyze and interpret data for presentation to key stakeholders.
• Team Collaboration: Collaborate closely with design team to determine protein constructs with optimal stability and functionality.
Qualifications (Minimal acceptable level of education, work experience, and competence)
• Advanced degree in chemistry, structural biology, biochemistry or related field with relevant experience in biotech or large Pharmaceutical company.
• Proven experience supporting drug discovery programs using structural biology and biochemistry.
• Good communication and interpersonal skills.
• Proven experience working with matrix teams
• Self-driven, creative and entrepreneurial with ability to support multiple programs simultaneously.
• Cryo-EM and Crystallography experience highly is desirable, but not required.
• Develop and implement short and long-term strategies to enhance protein production capabilities.
• Provide guidance on cloning, protein expression, purification, and biochemical and biophysical characterization
• Help troubleshooting recombinant protein expression and purification
• Protein engineering experience is desirable but not required
• May train, mentor, and supervise a team of scientists, technicians, and support staff
• Membrane protein experience is desirable but not required
• Knowledge of protein prediction and computational approaches for protein design
• Stay current on emerging technologies and instrumentation in protein chemistry and biochemistry to introduce innovative approaches to the facility
• Troubleshoot and optimize workflows to improve the efficiency and reproducibility of experimental results.
Disclaimer: The above statements are intended to describe the general nature and level of work performed by employees assigned to this job. They are not intended to be an exhaustive list of all duties, responsibilities, and qualifications. Management reserves the right to change or modify such duties as required.
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During the process, you may be asked to respond to questions that will screen out your application if you do not meet certain objective criteria required by the job. You can learn more about this process .
